SANAE FUKAYO

Approach to avoiding of accumulation of organic nitrogen in non-dilution mesophilic methane fermentation for food waste.

TAKASHI YAMAGUCHI

The food waste exhausted about 20 million tons or more in Japan. The recycling rate is low because the composition is not steady. Then, the methane fermentation process have been a focused as doesn't need strictĦĦsegregation of food wastes. Especially, the non-dilution methane fermentation method enables reducing effluent and making of the reactor capacity compact. However, when waste that in a high density contains nitrogen is processed, the methane fermentation might be obstructed with the ammonia generated by the degradation of proteins. In this study, it evaluated effect of pH for hydrolysis and convert organic-nitrogen into ammonia-nitrogen of food waste and researched flora in the reactors by 16SrRNA gene clone analyses for establish the non-dilution methane fermentation system for the food waste. The reactor's pH were controlled 7, 8, 9 after ammonia conversion rate reached more than 70% at pre-running. The ammonia conversion rate kept decreasing in all the pH running. One of the presumed cause is pH increase in the reactors that might damage to microbe. The ammonia-producing bacteria Peptostreptococcus appeared in all reactors and the percentage of sugar degradation bacteria Vagococcus were increased and microbial diversity were decreased with higher pH.

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