Ai MIYASHITA
Detection of anaerobic methane-oxidizing archaea, and attempt to cultivate with DHS reactor systems.
Takashi YAMAGUCHI
The anaerobic oxidation of methane (AOM) is mediated by anaerobic methane oxidizers falling into four 16S rRNA gene-based phylogenetic groups called anaerobic methanotroph (ANME). ANME archaea has not been cultivated yet, because of the growth rate is extremely slow and requires syntrophic association with sulfate / nitrate-reducing bacteria. The objective is to cultivate ANME archaea. Therefore, in this study we attempt to cultivate it by biological wastewater treatment system, down-flow hanging sponge (DHS) reactor.
First, we explored the sample to inoculate to the reactor. We collected from marine and terrestrial environments, but it is unclear in which terrestrial environments the ANME archaea exists. So we have designed specific primers for each ANME group and used to screen a variety of anaerobic environments. As a result, we detected clones belonging to ANME group from natural gas field and rice field.
Next, we attempt to cultivate ANME archaea by the reactor that inoculated marine sediments. During an incubation time of over 270 days, the sludge was retained in the reactor column. Furthermore, it occurred archaeal and bacterial activation. These results show the possibility that the ANME archaea can be cultured in the DHS reactor from now on
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